RBRC00983 <a href='https://brc.riken.jp/mus/pcr00983'>Genotyping protocol -PCR-</a> ホモノックインマウスは胎生致死(〜11.0)だが、ヘテロマウスは正常で繁殖に問題はない。 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in articles when the users publish their data using these mice for 2 year after provided. Users should contact the depositor in the case of application for any patents with the results from these mice. 山本 雅之 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="http://www.dmbc.med.tohoku.ac.jp/official/index.html" target="_blank">Lab HP (Japanese)</A> C(3〜6か月) C (3-6 months) E14 [129P2/OlaHsd] GATA-2 2nd Exon GFP knock in GATA-2 2nd Exon GFP knock in 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>寄託後2年間は共著を必要とする共同研究とする。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、寄託者と別途協議を行う。 E14ES (129Sv) 由来で、C57BL/6Jと交配。 Heterozygote x Wild-type [C57BL/6J] Homozygous mutat mice are embryonic lethal (E11.0). Fluorescent Proteins/lacZ System Developed by Drs. Naoko Minegishi and Masayuki Yamamoto, Center for Tukuba Advanced Research Alliance, University of Tsukuba in 2003. GFP was inserted in exon 2 of the Gata2 gene (Blood, 2003). Masayuki YAMAMOTO Heterozygote x Wild-type [C57BL/6J]Homozygous mutat mice are embryonic lethal (E11.0). true GFPの発現を指標にして、GATA-2発現細胞を同定できる。 Univ. Tsukuba B6;129P2-Gata2<tm1Mym>/Rbrc B6;129P2-Gata2<tm1Mym>/Rbrc B6;129P2-Gata2<tm1Mym>. GFP knock-in mice in the Gata2 locus, replacing the endogenous Gata2 coding sequences in the second exon. GFP expression was observed in the early hematopoietic tissues. Homozygous mutat mice are embryonic lethal (E11.0). Jellyfish GFP cDNA, mouse GATA-2 genomic DNA 国立大学法人筑波大学